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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Gene

51.1rMVA_108

Organism
Vaccinia virus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22mRNA capUniRule annotation1
Binding sitei39S-adenosyl-L-methionineUniRule annotation1
Binding sitei66S-adenosyl-L-methionineUniRule annotation1
Binding sitei68S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei72S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei95S-adenosyl-L-methionineUniRule annotation1
Binding sitei97S-adenosyl-L-methionineUniRule annotation1
Binding sitei116S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei138S-adenosyl-L-methionineUniRule annotation1
Active sitei175For methyltransferase activityUniRule annotation1
Binding sitei182mRNA capUniRule annotation1
Binding sitei233mRNA capUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionElongation factorImported, MethyltransferaseUniRule annotationImported, Transferase
Biological processmRNA cappingUniRule annotation, mRNA processing, Protein biosynthesis
LigandS-adenosyl-L-methionineUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferaseUniRule annotation (EC:2.1.1.57UniRule annotation)
Gene namesi
ORF Names:51.1rMVA_108Imported
OrganismiVaccinia virusImported
Taxonomic identifieri10245 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus

Interactioni

Subunit structurei

Methyltransferase activity: Monomer, poly(A) polymerase activity: Heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39.UniRule annotation

Structurei

3D structure databases

SMRiA0A2I6TDT0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni177 – 180mRNA cap bindingUniRule annotation4
Regioni205 – 207mRNA cap bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Poxvirus/kinetoplastid 2'-O-MTase family.PROSITE-ProRule annotation

Phylogenomic databases

KOiK21516

Family and domain databases

InterProiView protein in InterPro
IPR000176 mRNA_MeTrfase-like
IPR025804 Pox/kineto_cap_MeTfrase
IPR030375 Poxvir_cap_MeTfrase
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01358 PARP_regulatory, 1 hit
PIRSFiPIRSF003726 PolA_polym_reg_poxV, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51612 SAM_MT_2O_PK, 1 hit

Sequencei

Sequence statusi: Complete.

A0A2I6TDT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVVSLDKPF MYFEEIDNEL DYEPESANEV AKKLPYQGQL KLLLGELFFL
60 70 80 90 100
SKLQRHGILD GATVVYIGSA PGTHIRYLRD HFYNLGVIIK WMLIDGRHHD
110 120 130 140 150
PILNGLRDVT LVTRFVDEEY LRSIKKQLHP SKIILISDVR SKRGGNEPST
160 170 180 190 200
ADLLSNYALQ NVMISILNPV ASSLKWRCPF PDQWIKDFYI PHGNKMLQPF
210 220 230 240 250
APSYSAEMRL LSIYTGENMR LTRVTKSDAV NYEKKMYYLN KIVRNKVVVN
260 270 280 290 300
FDYPNQEYDY FHMYFMLRTV YCNKTFPTTK AKVLFLQQSI FRFLNIPTTS
310 320 330
TEKVSHEPIQ RKISSKNSMS KNRNSKRSVR SNK
Length:333
Mass (Da):38,888
Last modified:March 28, 2018 - v1
Checksum:i55A299DA4AAE1AD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
MG663594 Genomic DNA Translation: AUO38394.1
RefSeqiYP_232977.1, NC_006998.1

Genome annotation databases

GeneIDi3707551
KEGGivg:3707551

Similar proteinsi

Entry informationi

Entry nameiA0A2I6TDT0_9POXV
AccessioniPrimary (citable) accession number: A0A2I6TDT0
Entry historyiIntegrated into UniProtKB/TrEMBL: March 28, 2018
Last sequence update: March 28, 2018
Last modified: July 18, 2018
This is version 5 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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