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Protein

ADP-ribosylation factor 1

Gene

ARF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160GTP; via amide nitrogenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 32GTPCombined sourcesCurated9
Nucleotide bindingi126 – 129GTPCombined sourcesCurated4

GO - Molecular functioni

  • GTPase activity Source: Reactome
  • GTP binding Source: UniProtKB-KW
  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • RNA binding Source: UniProtKB
  • signal transducer activity, downstream of receptor Source: ProtInc

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-1660514 Synthesis of PIPs at the Golgi membrane
R-HSA-167590 Nef Mediated CD4 Down-regulation
R-HSA-2132295 MHC class II antigen presentation
R-HSA-421837 Clathrin derived vesicle budding
R-HSA-432720 Lysosome Vesicle Biogenesis
R-HSA-432722 Golgi Associated Vesicle Biogenesis
R-HSA-6807878 COPI-mediated anterograde transport
R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-HSA-6811438 Intra-Golgi traffic
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 1
Gene namesi
Name:ARF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi

Organism-specific databases

EuPathDBiHostDB:ENSG00000143761.13
HGNCiHGNC:652 ARF1
MIMi103180 gene
neXtProtiNX_P84077

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi375
OpenTargetsiENSG00000143761
PharmGKBiPA24934

Chemistry databases

ChEMBLiCHEMBL5985
DrugBankiDB04077 Glycerol
DB04121 Guanosine-3'-Monophosphate-5'-Diphosphate
DB04315 Guanosine-5'-Diphosphate
DB04137 Guanosine-5'-Triphosphate
DB08231 MYRISTIC ACID

Polymorphism and mutation databases

BioMutaiARF1
DMDMi51316985

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00002073782 – 181ADP-ribosylation factor 1Add BLAST180

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine; alternateCombined sources1
Lipidationi2N-myristoyl glycine; alternate4 Publications1

Post-translational modificationi

Demyristoylated by S.flexneri cysteine protease IpaJ which cleaves the peptide bond between N-myristoylated Gly-2 and Asn-3.2 Publications

Keywords - PTMi

Acetylation, Lipoprotein, Myristate

Proteomic databases

EPDiP84077
MaxQBiP84077
PaxDbiP84077
PeptideAtlasiP84077
PRIDEiP84077
TopDownProteomicsiP84077

PTM databases

iPTMnetiP84077
PhosphoSitePlusiP84077
SwissPalmiP84077

Expressioni

Gene expression databases

BgeeiENSG00000143761
CleanExiHS_ARF1
ExpressionAtlasiP84077 baseline and differential
GenevisibleiP84077 HS

Organism-specific databases

HPAiCAB007742

Interactioni

Subunit structurei

Interacts with ARHGAP21, ASAP2, GGA1, HERC1, PRKCABP, PIP5K1B, TMED2, PSCD2, TMED10 and GRIA2. Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Interacts with PI4KB in the Golgi complex. Interacts with NCS1/FREQ in the Golgi and at the plasma membrane. Interacts with PLEKHA3. Interacts with PLEKHA8; the interaction, together with phosphatidylinositol 4-phosphate binding, is required for FAPP2-mediated glucosylceramide transfer activity. Interacts (activated) with PICK1 (via PDZ domain); the interaction blocks Arp2/3 complex inhibition. Interacts with IQSEC1 (PubMed:24058294). Interacts with C9orf72 (By similarity).By similarity9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA

Protein-protein interaction databases

BioGridi106870, 99 interactors
DIPiDIP-31597N
ELMiP84077
IntActiP84077, 29 interactors
MINTiP84077
STRINGi9606.ENSP00000272102

Chemistry databases

BindingDBiP84077

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 9Combined sources4
Beta strandi10 – 12Combined sources3
Beta strandi14 – 16Combined sources3
Beta strandi19 – 25Combined sources7
Helixi30 – 36Combined sources7
Beta strandi42 – 45Combined sources4
Beta strandi49 – 51Combined sources3
Beta strandi53 – 58Combined sources6
Beta strandi61 – 67Combined sources7
Turni72 – 74Combined sources3
Helixi75 – 78Combined sources4
Helixi79 – 82Combined sources4
Beta strandi85 – 93Combined sources9
Helixi97 – 99Combined sources3
Helixi100 – 111Combined sources12
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Beta strandi131 – 134Combined sources4
Helixi136 – 143Combined sources8
Helixi145 – 147Combined sources3
Beta strandi153 – 157Combined sources5
Turni160 – 163Combined sources4
Helixi166 – 179Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HURX-ray2.00A/B2-181[»]
1RE0X-ray2.40A18-181[»]
1U81NMR-A18-181[»]
3O47X-ray2.80A/B11-181[»]
4HMYX-ray7.00C17-181[»]
6FAEX-ray2.35B18-181[»]
ProteinModelPortaliP84077
SMRiP84077
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP84077

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0070 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00780000121855
HOGENOMiHOG000163691
HOVERGENiHBG002073
InParanoidiP84077
KOiK07937
OMAiHYYSNTD
OrthoDBiEOG091G0S7S
PhylomeDBiP84077
TreeFamiTF300808

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR024156 Small_GTPase_ARF
IPR006689 Small_GTPase_ARF/SAR
PfamiView protein in Pfam
PF00025 Arf, 1 hit
PRINTSiPR00328 SAR1GTPBP
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51417 ARF, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P84077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K
Length:181
Mass (Da):20,697
Last modified:January 23, 2007 - v2
Checksum:iAAC773D4A60186B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36340 mRNA Translation: AAA35552.1
M84326 mRNA Translation: AAA35512.1
AF052179 mRNA Translation: AAC28623.1
AF055002 mRNA Translation: AAC09356.1
AF493881 mRNA Translation: AAM12595.1
BT007393 mRNA Translation: AAP36057.1
AL136379 Genomic DNA No translation available.
BC009247 mRNA Translation: AAH09247.1
BC010429 mRNA Translation: AAH10429.1
BC011358 mRNA Translation: AAH11358.1
M84332 Genomic DNA Translation: AAA35511.1
CCDSiCCDS1565.1
PIRiB40187 A33283
RefSeqiNP_001019397.1, NM_001024226.1
NP_001019398.1, NM_001024227.1
NP_001019399.1, NM_001024228.1
NP_001649.1, NM_001658.3
UniGeneiHs.286221

Genome annotation databases

EnsembliENST00000272102; ENSP00000272102; ENSG00000143761
ENST00000540651; ENSP00000442980; ENSG00000143761
ENST00000541182; ENSP00000440005; ENSG00000143761
GeneIDi375
KEGGihsa:375
UCSCiuc001hrr.4 human

Entry informationi

Entry nameiARF1_HUMAN
AccessioniPrimary (citable) accession number: P84077
Secondary accession number(s): P10947, P32889
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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