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Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

VACWR066

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves thiol-disulfide transfer between E10, A2.5 and G4.PROSITE-ProRule annotation1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
Gene namesi
Ordered Locus Names:VACWR066
ORF Names:E10R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

  • Virion membrane 1 Publication
  • Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).Curated

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8IntravirionSequence analysis8
Transmembranei9 – 25HelicalSequence analysisAdd BLAST17
Topological domaini26 – 95Virion surfaceSequence analysisAdd BLAST70

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000994651 – 95Probable FAD-linked sulfhydryl oxidase E10Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 95ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST95

Sequence similaritiesi

Belongs to the poxviridae E10 family.Curated

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090001G1

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
IPR006890 Sulphydryl_Oase_FAD-link_ERV1
PfamiView protein in Pfam
PF04805 Pox_E10, 1 hit
PIRSFiPIRSF015696 VAC_E10R, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

P23373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA
60 70 80 90
TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVT KVNPL
Length:95
Mass (Da):10,851
Last modified:November 1, 1991 - v1
Checksum:iF4A2FAB4159DFA50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36339 Genomic DNA Translation: AAB59830.1
AY243312 Genomic DNA Translation: AAO89345.1
PIRiT30803
RefSeqiYP_232948.1, NC_006998.1

Genome annotation databases

GeneIDi3707599
KEGGivg:3707599

Similar proteinsi

Entry informationi

Entry nameiE10_VACCW
AccessioniPrimary (citable) accession number: P23373
Secondary accession number(s): Q76ZV5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 25, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health