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Protein

Con-Ins G1

Gene
N/A
Organism
Conus geographus (Geography cone) (Nubecula geographus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This venom insulin facilitates prey capture by rapidly inducing hypoglycemic shock (PubMed:25605914, PubMed:27617429). It is the smallest known insulin found in nature and lacks the C-terminal segment of the B chain that, in human insulin, mediates engagement of the insulin receptor and assembly of the hormone's hexameric storage form (PubMed:27617429). Despite lacking this segment, it both binds and activates human insulin receptor (B isoform) with only a 10-fold reduced potency (PubMed:27617429). In vivo, intraperitoneal injection of this peptide into zebrafish lowers blood glucose with the same potency than human insulin (PubMed:25605914). In addition, when applied to water, this peptide reduces overall locomotor activity of zebrafish larvae, observed as a significant decrease in the percentage of time spent swimming and movement frequency (PubMed:25605914).2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHormone, Toxin
Biological processCarbohydrate metabolism, Glucose metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Con-Ins G11 Publication
Alternative name(s):
Insulin 1Imported
Cleaved into the following 2 chains:
Con-Ins G1 B chain1 Publication
Con-Ins G1 A chain1 Publication
OrganismiConus geographus (Geography cone) (Nubecula geographus)
Taxonomic identifieri6491 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000043929125 – 291 Publication5
PeptideiPRO_500211207230 – 52Con-Ins G1 B chain1 PublicationAdd BLAST23
PropeptideiPRO_000043929253 – 94C peptide1 PublicationAdd BLAST42
PeptideiPRO_000043929395 – 114Con-Ins G1 A chain1 PublicationAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei344-hydroxyproline; partial1 Publication1
Disulfide bondi38 ↔ 101Interchain (between B and A chains)Combined sources1 Publication
Modified residuei414-carboxyglutamate1 Publication1
Disulfide bondi50 ↔ 114Interchain (between B and A chains)Combined sources1 Publication
Modified residuei984-carboxyglutamate1 Publication1
Disulfide bondi100 ↔ 105Combined sources1 Publication
Modified residuei1044-hydroxyproline; partial1 Publication1
Modified residuei1094-carboxyglutamate; partial1 Publication1
Modified residuei114Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Gamma-carboxyglutamic acid, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Monomer (in solution).1 Publication

GO - Molecular functioni

Structurei

Secondary structure

1115
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi96 – 100Combined sources5
Turni101 – 104Combined sources4
Helixi107 – 110Combined sources4
Helixi111 – 113Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JYQX-ray1.95A95-114[»]
B30-52[»]
SMRiA0A0B5AC95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the insulin family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR016179. Insulin-like.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
PfamiView protein in Pfam
PF00049. Insulin. 1 hit.
PRINTSiPR00276. INSULINFAMLY.
SMARTiView protein in SMART
SM00078. IlGF. 1 hit.
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiView protein in PROSITE
PS00262. INSULIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0A0B5AC95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSSYFLLM ALGLLLYVCQ SSFGNQHTRT FDTPKHRCGS EITNSYMDLC
60 70 80 90 100
YRKRNDAGEK RGRASPLWQR RGSLSKLKAR AKRNGAFHLP RDGRGVVEHC
110
CHRPCSNAEF KKYCG
Length:115
Mass (Da):13,134
Last modified:April 1, 2015 - v1
Checksum:iF578697A311DBB23
GO

Mass spectrometryi

Molecular mass is 5030.1 Da from positions 30 - 52 and 95 - 114. Determined by ESI. with 1 hydroxyPro (residue 34) and with 3 carboxyglutamate (residues 41, 98 and 109).1 Publication
Molecular mass is 5046.1 Da from positions 30 - 52 and 95 - 114. Determined by ESI. with 2 hydroxyPro (residues 34 and 104) and with 3 carboxyglutamate (residues 41, 98 and 109).1 Publication
Molecular mass is 5014.1 Da from positions 30 - 52 and 95 - 114. Determined by ESI. without hydroxyPro and with 3 carboxyglutamate (residues 41, 98 and 109).1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KP268599 mRNA. Translation: AJD85832.1.

Similar proteinsi

Entry informationi

Entry nameiINS1A_CONGE
AccessioniPrimary (citable) accession number: A0A0B5AC95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
Last sequence update: April 1, 2015
Last modified: August 30, 2017
This is version 15 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families